Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2018 Aug 22;7(9):bio035568. doi: 10.1242/bio.035568

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© 2018. Published by The Company of Biologists Ltd

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/3.0), which permits unrestricted use, distribution and reproduction in any medium provided that the original work is properly attributed.

PMC Copyright notice

Fig. 1. — Directed differentiation of hiPSCs to sensory neurons under CXCR4 stimulation or inhibition. Differentiation cultures from four time-points (4, 8, 12 and 15 days) were cultured, each with three groups [control, inhibitor (AMD3100) and agonist (SDF1)] with six experimental replications each (72 cultures total). (A,B) Transcription level of various markers relative to GAPDH in the three differentiation groups on days 4 and 8 shows the transition to neural crest cells (TFAP2A/SOX10). Grouping neural crest markers shows their expression to be not significantly (NS) higher than non-neural crest markers on day 4, but highly significant (****P<0.0001) by day 8. (C,D) expression of TFAP2A and SOX10 in the three groups relative to GAPDH over four time-points (day 4, 8, 12 and 15). (E–G) Sensory neuron differentiation progression of the control group with nuclei in blue, neurites (beta-III-tubulin) in green, and TrkA in red (sensory neurons, G only) on days 8, 12 and 15. (H) Transcription levels of markers relevant to sensory neuron differentiation and subtype specification relative to GAPDH on day 15. (I) Transcription levels of TrkA and TrkB in the three groups relative to GAPDH on day 15. qPCR: single cDNA pool from two replications, error bars: standard deviation of qPCR replications. Scale bar: 150 µm.