Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2018 Aug 22;7(9):bio035568. doi: 10.1242/bio.035568

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© 2018. Published by The Company of Biologists Ltd

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/3.0), which permits unrestricted use, distribution and reproduction in any medium provided that the original work is properly attributed.

PMC Copyright notice

Fig. 2. — Culture morphology and cell adhesion molecule expression analysis after CXCR4 stimulation or inhibition. (A,B,C) Day 15 cultures from the control, SDF1 (agonist) and AMD3100 (antagonist) groups stained for TrkA (red, nociceptive marker), nuclei (blue) and beta-III-tubulin (green, neurite marker). (D,E,F) Day 15 cultures from the Control, SDF1 and AMD3100 groups stained for TrkB (red, mechanoreceptive marker), nuceli (blue) and beta-III-tubulin (green, neurite marker). Control (with endogenous SDF1) and SDF1-stimulated differentiation cultures develop into DRG-like clusters of radially projecting nociceptive neurons (arrows), whereas inhibitory conditions result in a more evenly distributed phenotype when looking at beta-III-tubulin staining (N=3 per condition, none excluded). (G) Comparison of transcript levels of various cell adhesion proteins between the differentiation groups relative to GAPDH (Single cDNA pool from two replications). Error bars: standard deviation of qPCR replications. Scale bar: 150 µm.