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Yolk syncytial layer accumulation of unesterified cholesterol. Plasmids expressing either EGFP or npc1 were injected into the yolk syncytial layer (YSL) of 3.5 hpf embryos. Filipin staining of 2 dpf embryos demonstrated reversal of unesterified cholesterol accumulation in the YSL of mutant larvae expressing npc1. Scale bar: 200 μm.
