Fig. 5.

Microglial accumulation of autofluorescent material in white light-exposed CLN3-deficient mice. (A) Some Iba1-positive cells are already observable in the subretinal space 1 day after light exposure in CLN3-deficient retinas exposed to ‘low light’ and ‘high light’ conditions as well as in wild-type mice after exposure to ‘high light’ conditions. Only a few microglia are found in the subretinal space of wild-type mice after ‘low light’ conditions at all three time points. An increased amount of autofluorescent material was present 4 and 7 days after light exposure in CLN3-deficient retinas under ‘low light’ conditions and for both genotypes under ‘high light’ conditions. Scale bar: 100 µm. (B-D) Quantification of the Iba1-positive area (B), the number of microglial (C) and the area covered by autofluorescent material (D) show highly significant differences between CLN3-deficient and wild-type mice treated with ‘low light’ conditions. Data show mean±s.e.m. (n=3 mice/group) from two independent experiments with ***P<0.001. AF, autofluorescence.