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. 2018 Sep 10;11(9):dmm033704. doi: 10.1242/dmm.033704

Fig. 3.

Fig. 3.

Mutated M1, but not M85, decorates a subset of MTs in mouse cortical neurons. (A) Mouse cortical neurons at 4 DIV were transfected with GFP-tagged spastin mutants, fixed after 16 h and stained for acetylated tubulin. Magnifications show the MT pattern for M1CY and discontinuous MT decoration for M1Δ. (B) Mouse cortical neurons were transfected with M1CY-Flag and M85CY-GFP, fixed and stained for β-tubulin (not shown in the overlay). Scale bars: 20 µm.