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. 2018 Sep 13;11(9):dmm033449. doi: 10.1242/dmm.033449

Fig. 2.

Fig. 2.

Immuno-verification and real-time PCR analysis of mutant Dictyostelium strains. (A) Verification of generated D. discoideum strains by western blot analysis with anti-strumpellin and anti-GFP antibodies. The asterisk indicates an unspecific band. (B) Real-time PCR analysis of AX2 WT and mutant strains. The relative mRNA expression levels of the genes encoding strumpellin, p97, members of the WASH complex, ARPC4 and Cap32/34 in D. discoideum cells are depicted. The expression level in AX2 was set to 1. Means±s.d. of three independent determinations are depicted. (C) Immunoblot analyses of SWIP, p97 and Cap32 in the generated D. discoideum strains using specific antibodies. Actin (lower panel) served as a loading control. (D) Quantitation of SWIP expression in AX2 WT and Str− cells. Means±s.e.m. of three independent experiments are depicted. Significance was calculated using Student’s t-test. *P≤0.05.