Fig. 5.

Cancer modeling with TEAZ enables sequential electroporation of transgenes. A tumor-bearing fish (created with rb1 sgRNA as in Fig. 2) was imaged using GFP and tdTomato. As expected, only GFP⁺ tumor cells were seen, with no expression in the tdTomato channel. This tumor was then electroporated with an mitfa:tdTomato plasmid and re-imaged 5 days later, showing areas that are now both GFP⁺ and tdTomato⁺ (n=2).