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. 2018 Sep 27;14(9):e1007697. doi: 10.1371/journal.pgen.1007697

Fig 5. Tnks DKO leads to decreased Wnt/β-catenin signaling activity.

Fig 5

(A) Crypts were isolated from the mice (n = 3) at day 3 and day 4 after the first TAM injection for qRT-PCR analysis to detect the expression of Wnt targets. Data represent mean ± SD, analyzed by two-way ANOVA test. *P<0.05 and **P<0.01. (B) Immunoblotting analysis of isolated small intestine crypts from control and DKO mice. GAPDH serves as an internal control. The representative result of three independent experiments was shown here. (C) Crypts were isolated from control and DKO mice were at day 4 after first TAM injection for immunoprecipitation (IP) with control IgG or anti-PAR antibody followed by immunoblotting with indicated antibodies. The representative result of three independent experiments was shown here. Protein expression was determined with whole-cell lysates.