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. 2018 Sep 27;14(9):e1007483. doi: 10.1371/journal.pgen.1007483

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© 2018 Dai et al

This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

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Fig 4 — (A) Confocal images of the larvae fat body BM showing localization of Laminin (LanB1.sGFP, cyan), Collagen IV (Vkg.GFP, green) and Perlecan (Trol.YFP, yellow) from control (upper panels), Ndg¹ mutant (middle panels) and larvae where Ndg has been knocked down under control of Cg-GAL4 (Ndgⁱ, lower panels). Loss of Ndg causes discontinuity of adipose tissue BMs. (B) Discontinuous BMs (Vkg.GFP, green) in the larval fat body of transheterozygotes Ndg¹/Ndg² (upper panel), Ndg¹/Ndg^ΔRod-G3.1 (middle panel) and Ndg¹/Ndg^Df(2R)BSC281 (lower panel). (C) Fat body BM (Cg25C.RFP, red) in wild type, Ndg¹ mutant and Ndg¹ mutant rescued with Ndg.sGFP. (D) Lipid droplets (neutral lipid dye BODIPY, green) in fat body of wild type (upper panel), Ndg¹ mutant (middle panel) and Ndg² mutant (lower panel) larvae. Asterisks point to cells with reduced content of lipid droplets. Nuclei stained with DAPI (blue). Scale bar represents 50μm (A-D). (E) Quantification of lipid droplet diameter in 16 cells from wild type control, Ndg¹ and Ndg² mutants. Each dot represents a single droplet. Particles smaller than 3μm in diameter were excluded from the analysis. Horizontal lines indicate the mean value and error bars represent ±SD. Difference with the wild type are significant in non-parametric Mann-Whitney tests (****: p<0.0001).