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. Author manuscript; available in PMC: 2019 Sep 10.
Published in final edited form as: Dev Cell. 2018 Sep 10;46(5):595–610.e3. doi: 10.1016/j.devcel.2018.08.009

Figure 2: WDR5 mutants localize near the base of cilia and nucleus in MCCs.

Figure 2:

(A) WDR5 was tagged with 3×GFP at the C-terminal end to study the localization of WDR5 mutants at the ciliary base. Wildtype WDR5 was localized to the nucleus and ciliary base in MCCs. None of the WDR5 deletion constructs (1–117, 1–201, 118–334, 118–201, and 202–334) localized either to the nucleus or ciliary base.

(B) Mutants 26–334 WDR5, patient mutation (K7Q) and methylation dead mutant (S91K) localize at the ciliary base and nucleus. Control 3×GFP only does not localize specifically to either location.

See also Figure S4