Figure 5. Myeloid Defects Are Associated with Goblet Cell Alterations.

(A) Quantification of bacterial penetration into the colonic lamina propria of Commd1^fl/fl or Commd1^ΔMye, expressed as fold over the Commd1^fl/fl group (based on FISH staining). Additional data are presented in Figures S4A and S4D. Data displayed are mean and SEM.

(B) Representative images of Alcian blue staining of the distal colon of Commd1^fl/fl or Commd1^ΔMye mice (scale bars, 100 μm). Additional data are presented in Figures S4B and S4C.

(C) Quantification of goblet cell density in the distal colon of Commd1^fl/fl or Commd1^ΔMye (5 mice per group). Data displayed indicate mean and SEM. Additional data are presented in Figure S4E.

(D) Same as in (A), but in Commd9^fl/fl or Commd9^ΔMye mice (6 animals per group).

(E) Same as in (B), but in Commd9^fl/fl or Commd9^ΔMye mice.

(F) Same as in (C), but in Commd9^fl/fl or Commd9^ΔMye mice (6 animals per group).

(G) Same as in (A), but in Cybb⁺ (n = 4) or Cybb⁻ (n = 5) mice.

(H) Same as in (B), but in Cybb⁺ or Cybb- mice.

(I) Same as in (C), but in Cybb⁺ or Cybb⁻ mice (5 animals per group).

(J) Representative images of immunofluorescence staining for Muc2 (red), UAE-1 lectin (green), and nuclei (Hoechst, blue) in colonic tissue (from over 3 separateiterations). Scale bars, 100 μm (50 μm in insets).

(K) Representative images (from over 5 separate iterations) of fluorescence in situ hybridization (FISH) for eubacteria in colonic tissue (treated with Carnoy’s fixative to preserve the mucus layer). Scale bars, 50 μm.

(L) Quantification of the distance between the top of the epithelium and luminal bacteria is depicted in Commd1^fl/fl or Commd1^ΔMye mice (5 animals per group).Data displayed are mean and SEM. *p < 0.05; **p < 0.01; ns, not significant (unpaired Student’s t test). Figure S4.