Figure 2.

Discovery of cross-reactive α-NanBC mAbs. (A) Conservation among NanA, NanB and NanC. NanA structure as depicted in Figure 1, with residues identical in NanB (left panel) and NanC (4YC4 [27], middle panel) marked in black, and NanB (PDB 2VW1 [25], right panel, colors as for NanA) with residues identical in NanC marked in black. (B) Mouse hybridoma mixed clone supernatants tested for neutralization of recombinant NanB and NanC (2 nM each) in a fluorometric screening assay. One representative experiment is shown. (C) Three human-mouse chimeric mAbs expressed in CHO cells, tested at the indicated concentrations for inhibition of NanB and NanC in two independent experiments shown as mean with standard deviation.