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Contribution of individual Nans to the total neuraminidase enzymatic activity differs among S. pneumoniae strains. Neuraminidase activity of recombinant neuraminidases (200 pM each) (A) or pneumococcal stationary phase CSs (B) in the presence of α-neuraminidase mAbs (1 µM each). Two clones per strain were used for CS generation. Percent inhibition was calculated relative to a negative control mAb. Results from two independent experiments are shown as mean with standard deviation.
