Figure 4.

Multiple neuraminidases contribute to desialylation of primary human lung tissue by S. pneumoniae. Primary human lung tissue treated with an equimolar mixture of all three recombinant neuraminidases (5 nM each) ± α-neuraminidase mAbs (2 µM each) (A) or infected with S. pneumoniae D39Δply +/- α-neuraminidase mAbs (2 µM each) or control mAb (4 µM) (B) for 24 h. Desialylation was visualized by Peanut Lectin-FITC staining (green). Nuclei were stained with Hoechst dye (blue). Representative images of one experiment with biological duplicates are shown. Scale bar = 50 μm.