Table 1.
PCR primers used in this study.
| Designation | Sequence (5ʹ to 3ʹ) |
|---|---|
| For deletional mutagenesis | |
| ccs4KOuF | AGCTTATCCCGACCTTCTTTCTG |
| ccs4KOuR | GTATTCAAATATATCCATCGTTTCTCCTGTTCAATTTATC |
| ccs4KOdF | ATTATAAAAAAATTGATACTGCGACTCTTTATCGTAAGAG |
| ccs4KOdR | GGGTGTCACATCCATAACCTTG |
| ccs4KOaad9F | GAACAGGAGAAACGATGGATATATTTGAATACATACGAAC |
| ccs4KOaad9R | TAAAGAGTCGCAGTATCAATTTTTTTATAATTTTTTTAATC |
| For construction of plasmid used for ectopic expression of Ccs4 | |
| Ccs4F+ pDCerm | GTTGCATGCGGTACCATGAGTGTGTATGGTAGAGTAGAAG |
| Ccs4R+ pDCerm | GCGCATGCTAAGCTTTCATCCCATGCATGAGATAGCATCT |
| pDCermF+ Ccs4 | TCATGCATGGGATGAAAGCTTAGCATGCGCTGAAGCGAAG |
| pDCermR+ Ccs4 | ACCATACACACTCATGGTACCGCATGCAACCTCTGTTTGT |
| pDCermUinivF | GAAGAAAAGAGCTTTGCTAGG |
| pDCermUinivR | ATCTCCAATCATAAAAATAAC |
| Real-time RT-PCR primers | |
| TIGR4ccs4F | TCCTTTCTTATTCGCAGCCTTT |
| TIGR4ccs4R | CCTTATCATGGCTTGGATTGC |
| TIGR416srRNAF | TGTAGCGGTGAAATGCGTAGATA |
| TIGR416srRNAR | CAAGCCAGAGAGCCGCTTT |
| TIGR4nanAF | ATTTGACCCCCAAACCGTATT |
| TIGR4nanAR | ATGTAACGCATGTGCAAATCG |
| TIGR4cbpAF | CGACCTCTTTCCTTGCCTTATC |
| TIGR4cbpAR | GGCAGAACAAGGAGAACAACCT |