Figure 10.
IgM Western blot analysis of cerebrospinal fluid (CSF) of experimentally infected piglets with acute meningitis and high intracerebrospinal S. suis burden reveals only intact IgM. Piglets were intranasally infected with the indicated strains and the CSF of one pig with meningitis of each infection group was analyzed for the presence of IgM cleavage products by anti-IgM Western Blot analysis under non-reducing conditions with a monoclonal anti-IgM antibody. Protein concentrations in the CSF were adjusted before loading the 6% polyacrylamide gel. As a control (lanes 7–10), CSF of the same piglets was incubated with 5 µg/ml rIdeSsuis. Asterisks indicate IgM cleavage products for the last positive lane. Marker bands (in kDa) are shown on the left-hand side.