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. 2018 Sep 21;7:e35316. doi: 10.7554/eLife.35316

Video 11. Hypoxia stimulates TRPA1 sparklet frequency in the cerebral endothelium.

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DOI: 10.7554/eLife.35316.029

Representative movie of the same field of view from Video 10 showing that superfusing the preparation with hypoxic (5% O2, 6% CO2, 89% N2), warm (37°C) physiological saline solution (PSS) increases the frequency and number of active sites of TRPA1 sparklets. TRPA1 sparklets were recorded in the presence of EGTA-AM (10 µM) and CPA (30 µM).