Figure 5. Actin, proteasome and mitochondrial network reorganization.

WT cells expressing Ilv3-RFP and Abp1-3xGFP (actin) or Scl1-GFP (proteasome) were grown 7 d in YPD and refed on a YPD microscope pad. (A) Histograms show the percentage of individual cells displaying AB or PSG able to form a bud within 6 hr after re-feeding. The theoretical ‘independence’ value (diamond) and representative image series are shown; n > 200, N = 2. (B) Percentage of individual cells able to form a bud among cells with both a vesicular mitochondrial network and AB or PSG. Representative image series are shown; n > 300, N = 2. (C) Actin and proteasome organization in cells within vesicular and globular mitochondria cell populations; n > 300 N = 2. (D) Mitochondria organization in cells with AB or PSG; n > 450 and>300 respectively, N = 2. Bars are 2 μm. (E) Cell type and fate distribution among a WT population grown for 7 d. Vesicular mitochondria (small red dots), globular mitochondria (big red dots), AB (big blue dot) and PSG (green dot) are schematized.

Figure 5—figure supplement 1. Actin and Proteasome localization in WT and rho zero cells, cell volume variation according to mitochondrial network organization.

(A) Actin phalloidin staining of WT prototroph rho zero cells after 24 hr of culture in YPD. The image is a superposition of the DIC and the RFP channel. (B) Proteasome (Scl1-GFP) localization in WT and WT rho zero cells after 6 d in YPD. Bars are 2 μm. (C) Cell volume of daughter, mother and old mother (>5 bud scars) cells after 7 d in YPD according to their mitochondria organization. The median cell volume and the percentage of cells with globular mitochondria (in red) within each sub-population are indicated.