Representative images of HRP extravasation after FPI with injured and sham groups of 1 h (A-B), 3 h (C-D), 6 h (E-F). HRP was injected ten mins before sacrifice. Quantification of HRP extravasation after FPI. (ii) Cortex, (iii) Corpus callosum. * P < 0.05 compared to respective sham group, two-way ANOVA, Bonferroni’s post-hoc test. # P < 0.05 compared to 1 h and 6 h injured cohort, two-way ANOVA, Tukey’s post-hoc test. Two-segmented y-axis is used to display the sham groups. Error bars represent standard error of mean with n = 3. Scale bars = 200 μm.