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. Author manuscript; available in PMC: 2018 Oct 9.
Published in final edited form as: Biochemistry. 2018 Sep 24;57(40):5827–5840. doi: 10.1021/acs.biochem.8b00650

Table 4.

ET Rate Constants of Met80-ligated Yeast Iso-1 Cyt c Variants

variant kET (mM−1s−1) kESE (mM−1s−1)
0.1 M NaCl 1 M NaCl
WTa 48 ± 4b n.a. n.a.
WT* 37.1 ± 1.0 261.8 ± 21.5 1.28 ± 0.22
K79G 381.8 ± 19.9 742.8 ± 30.6 20.8 ± 9.04
K73Ha 39 ± 5b n.a. n.a.
K72A/K73H 47 ± 1c n.a. n.a.
K73H/K79Aa 133 ± 9d n.a. n.a.
N52G/K79Aa 160 ± 40d n.a. n.a.
a

These variants contain tmK72 and a background C102S mutation. The rest of the variants contain K72A and C102S mutations.

b

From ref 75.

c

From ref 76.

d

From ref 25.