Table 4.
ET Rate Constants of Met80-ligated Yeast Iso-1 Cyt c Variants
variant | kET (mM−1s−1) | kESE (mM−1s−1) | |
---|---|---|---|
0.1 M NaCl | 1 M NaCl | ||
WTa | 48 ± 4b | n.a. | n.a. |
WT* | 37.1 ± 1.0 | 261.8 ± 21.5 | 1.28 ± 0.22 |
K79G | 381.8 ± 19.9 | 742.8 ± 30.6 | 20.8 ± 9.04 |
K73Ha | 39 ± 5b | n.a. | n.a. |
K72A/K73H | 47 ± 1c | n.a. | n.a. |
K73H/K79Aa | 133 ± 9d | n.a. | n.a. |
N52G/K79Aa | 160 ± 40d | n.a. | n.a. |
These variants contain tmK72 and a background C102S mutation. The rest of the variants contain K72A and C102S mutations.
From ref 75.
From ref 76.
From ref 25.