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. 2018 Oct 4;13:6019–6028. doi: 10.2147/IJN.S175523

Figure 1.

Figure 1

(A) Gold nanoparticle (AuNP) characterization: ultraviolet-visible spectroscopy (UV-Vis) spectrum of 80 nm diameter gold nanospheres (AuNS) compared with Mie theory and finite-difference time-domain (FDTD) simulations. The UV-Vis spectrum is the ensemble spectrum which is slightly red shifted compared to Mie theory and FDTD simulated single particle spectrum. (B) Typical confocal laser scattering point spread function (PSF) profile calculated from the cross-section of one of the areas of scattered intensity and collected with a photomultiplier tube, when the laser is scanned across the sample. The analytical PSF (blue continuous line) from single particles matches perfectly with experimental PSF (red dotted line), whereas the dimer has 1.2 times higher PSF (black dotted line). Red color spectrum indicates analytical PSF using Vectorial Debye theory for objective 1.4 numerical aperture, at 715 nm wavelength for circular polarization. Vectorial Debye theory can be used to calculate the diffraction pattern of an objective lens of high numerical aperture.