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. 2018 Oct 9;8:14987. doi: 10.1038/s41598-018-32889-y

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Clinical outcome in animals given retrorsine. (a–c) H & E staining of liver from (a) a pig receiving 30 mg/kg RS, dying 3 days after the first RS injection, (b) a pig receiving 50 mg/kg RS, dying 26 days after the second RS injection and (c) a pig receiving 100 mg/kg RS, dying on the day after the second injection of RS. (d) Kaplan-Meier survival curves for follow-up of 21 pigs after the first injection of RS. (e,f) Clinical scores of appetite (e) and activity (f) of pigs after 0 or 50 mg/kg RS treatment (mean + SD). ^*P < 0.05.