Fig. 4. Upregulation of NF-κB target gene protein expression in MWCL-A20_ko and HBL-1-A20_ko cells.

a Western blot analysis of NF-κB target genes BCL2 and MYC in MWCL (BCL2, n = 4; MYC, n = 3) and HBL-1 (BCL2, n = 3; MYC, n = 3) A20 knock out cell lines and their wild type counterpart β-actin was used as a loading control. b Upregulation of IL-6 and CXCL10 secretion in MWCL (n = 3) and HBL-1 (n = 3) A20 knock out cell lines and their wild type counterpart. The representative experiment is shown and cytokine assay was run in duplicates. Each bar represents the mean values of expression levels ± SD. *p ≤ 0.05, **p ≤ 0.01