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. 2018 Oct 9;9(10):1027. doi: 10.1038/s41419-018-1036-5

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 2 — a Co-immunoprecipitation analysis of the interaction between AFP and PTEN in AFP-siRNA923 (AFP-si)-transfected PLC/PRF/5 cells. b Western blotting for the effects of AFP knockdown on the expression of PI3K/AKT/mTOR signalling-related proteins in AFP-si-transfected cells. c Co-immunoprecipitation analysis of the interaction between AFP and PTEN in pcDNA3.1-afp (V-afp)-transfected HLE cells. d. Western blotting for the effects of AFP overexpression on the expression of PI3K/AKT/mTOR signalling-related proteins in HLE cells. Images are representative of at least three independent experiments