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. 2018 Oct 9;8:15009. doi: 10.1038/s41598-018-33391-1

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Heat map of polyketide synthase gene expression in C. rosea. (A) During mycelial growth in liquid CZ, PDB and SNB culture media. C. rosea mycelia grown in CZ medium were used as control treatment. (B) During interactions with itself (Cr-Cr), B. cinerea (Cr-Bc) or F. graminearum (Cr-Fg). C. rosea interaction with self (Cr–Cr) was used as control treatment. (C) During pigment production. Four days old C. rosea culture plates, incubated at the same conditions were used as control treatment as no pigmentation was observed at this stage. Relative expression level based on RT-qPCR was calculated as the ratio between the target PKS gene and β-tubulin using 2^−ΔΔCt method (Livak and Schmittgen, 2001), and compared with the respective control. Statistically significant differences (P ≤ 0.05) in gene expression between treatments were determined using Fisher’s exact test and are indicated by different letters. The scale representing the relative expression intensity values is shown.