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. 2018 Oct 9;8:15009. doi: 10.1038/s41598-018-33391-1

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Antifungal activity of compound 1 and compound 2 extracted from C. rosea against plant pathogens B. cinerea and F. graminearum. Conidial suspensions in half-strength potato dextrose broth were inoculated in 96-well microtiter plates containing compound 1 or compound 2. Frequency of germinating conidia was determined 8 hour post-inoculation by counting the number of germinating and non-germinating conidia, while germ tube length was measured using ImageJ software. (A) B. cinerea conidia germination, (B) B. cinerea germ tube length, (C) F. graminearum germ tube length. Abbreviation: 1, compound 1; 2, compound 2. Concentrations of compound are indicated below the bar. Error bars represent standard deviation based on three biological replicates. Different letters indicate statistically significant differences (P ≤ 0.05) within the experiments based on Fisher’s exact test.