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. 2018 Oct 9;8:15029. doi: 10.1038/s41598-018-33239-8

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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The generation of the stable overexpression and knockdown cell lines. (a) The expression of CHI3L1 in the HepG2, HepG2.2.15, Bel7404, MHCC97L and HCCLM3 by Two-step RT-PCR and the grayscale of the strips were showed. (b) Immunofluorescence was showed CHI3L1 overexpression in HepG2 and Bel7404 cells. Cell nucleus was stained with DAPI (blue fluorescence) and CHI3L1 was stained with FITC (green fluorescence). (c) The expression of CHI3L1 in the HepG2 and Bel7404 cells after transfected with three short-hairpin RNAs. All samples were normalized to the housekeeping gene GAPDH. (d) qPCR showing the expression of CHI3L1 in the CHI3L1 overexpression and knockdown cell lines. Scale bar represents 100 μm. Error bars represents SD calculated from three independent experiments. **Represents p < 0.01 by two-tailed t test.