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. 2018 Oct 9;8:15029. doi: 10.1038/s41598-018-33239-8

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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CHI3L1 promotes cancer cell migration and invasion in vitro. (a) Eight stable transfected cell lines (HepG2-CHI3L1, HepG2-Con, HepG2-shCHI3L1, HepG2-Shc; Bel7404-CHI3L1, Bel7404-Con, Bel7404-shCHI3L1, Bel7404-Shc) were seeded into the upper chambers of transwell plates and complete culture medium was added to the lower chambers. Numbers of migration cells were counted and image were taken at 10 hours after seeding the cells. (b) The invasion assays were performed on these 8 stable transduced cell lines. Numbers of invasion cells were counted and image were taken at 48 hours after seeding the cells. (c) Cells were seeding into 6-wells plates. The images were taken at 48 hours after the scratches were made. **, *** and **** represent p < 0.05, p < 0.01 and p < 0.001 by two-tailed t test. Scale bar represents 50 μm. Error bar represents SD calculated from three independent experiments.