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. 2018 Oct 9;8:15024. doi: 10.1038/s41598-018-32929-7

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Reversibility and versatility of the Blue-OFF system. (a) HEK-293T cells were transfected with KRAB-EL222 (pKM565) and FLuc-B-LID (pMZ1203).and kept in darkness for 12 h followed by 12 h blue light illumination and again 12 h darkness. FLuc levels were measured every 2 h. (b) Blue-OFF characterization using different mammalian cell lines. The indicated cell lines were transfected with KRAB-EL222 and FLuc-B-LID. Cells were kept in darkness for 16 h followed by 8 h of 460 nm blue light illumination. FLuc levels were determined at the final time point. To correct for different transfection efficiencies, the expression data were normalized to co-transfected constitutively expressed Renilla luciferase (RLuc). In a and b, data are means of four independent replicates and error bars indicate standard deviation of the mean.