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. 2018 Oct 9;8:15034. doi: 10.1038/s41598-018-33102-w

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Co-localization analysis of SpiroZin2 and three vesicular markers demonstrates that SpiroZin2 resides in lysosomal vesicles. (a) Representative images of SpiroZin2-stained cells, vesicular markers- FP, and merged channel. (b) Pearson coefficient plot of co-localization analysis. Pearson analysis included a minimum of 21 cells per condition. Scale bar, 5 μm.