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. 2018 Oct 9;8:15005. doi: 10.1038/s41598-018-33379-x

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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KSHV ORF57 residues 68–178 interaction with UIF and ALYREF mapped by NMR. (A) Perturbations in ¹⁵N HSQC spectra were used to map interactions of ¹⁵N-labeled ksORF57^68–178 with unlabeled constructs: (i) ksORF57^68–178 with REF^1–155 (free in red, bound in green), (ii) ksORF57^68–178 with UIF^NT (free in red, bound in blue). (B) Signal shifts and intensity perturbations induced by REF and UIF mapped onto the sequence of ksORF57 from analysis of spectra in panel A. Broadened residues indicated by circles and moderate and large shifts indicated by blocks.