Fig. 6. EtOH sensitizes HepG2 cells to tBid-induced OMMP.

a Cell viability was tested for 100 mM EtOH and/or 30μM Bid BH3_cp-treated intact HepG2 cells (n = 4, *p < 0.05). b Time-course recording of ΔΨ_m in permeabilized HepG2 cell suspension using fluorometer. 0, 100 nM tBid was added at 500 s. Red traces represent EtOH-treated cells and black traces non-treated ones. c Similar to b except lower concentrations of tBid (0, 2.5, 0.5, and 0.25 nM) were used (n = 3). d Dose–response relationship between tBid concentration and ΔΨ_m dissipation in HepG2 permeabilized cells. e Immunoblot of cyto c in the cytosolic fraction of the permeabilized cells that were separated quickly from pellet fraction right after 300 s treatment with tBid or solvent. Cyto c was loaded as a positive control. f Similar to e except the treatment times were either 300 s or 150 s