Figure 3.
Validation of lncRNA and mRNA microarray data by qRT-PCR. RNA from mouse BMDM were collected 6 hr after infection with either RH or PTG strains of T. gondii, and qRT-PCR was subsequently performed. Fold changes represent the comparison of infected samples to uninfected samples. Regulation of representative lncRNAs as determined by qPCR (a) and microarray (b) analysis. Regulation of immune-related mRNA is shown by qPCR (c) and microarray (d) analysis. Microarray fold change values differ slightly from those listed in Supplementary Spreadsheets 1 and 2 (which are the geometric means) because arithmetic means were calculated from the microarray data to directly compare to qRT-PCR data. Experiments were completed a minimum of three times with BMDM from three separate mice and were obtained independently of experiments used for microarray analysis.