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. 2018 Oct 9;8:14997. doi: 10.1038/s41598-018-33387-x

Figure 1.

Figure 1

Math6 gene targeting strategy. (A) Schematic drawing of the Math6 WT allele, the gene targeting vector, the recombined Math6 allele and the alleles resulting from the sequential deletion of the Neo-cassette (Math6 flox allele) and Math6 exon 1 (Math6 KO allele) by Flp- and Cre-excision respectively. The long homology arm upstream of the 5′-flanking loxP-site is 5.9 kbp, whereas the short homology arm downstream of the 3′-flanking loxP site is 1.6 kbp in length. With respect to the Math6 WT allele, the 5′-flanking loxP-site is located at position −1251 to −1217 with respect to the beginning of exon 1 (+1), whereas the 3′-loxP site is located at position +1389 to +1423. The locations of the DNA probe and PCR primers used for the detection of a successful gene targeting are indicated. (B) Southern Blot analysis of genomic DNA reveals the occurrence of all three Math6 genotypes in the offspring of Math6+/− intercrosses (WT allele: 7.2 kbp/KO allele: 4.7 kbp; picture shows cropped lanes of one blot cp. Fig. S8).