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. 2018 Oct 9;8:15022. doi: 10.1038/s41598-018-33358-2

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Febrile temperature greatly enhanced cell-cell adhesion, and the contact diameter between cells. (A) Schematic illustration of the geometry of one iRBC (left) being separated from the CHO cell (right), and the calculation of adhesion force and energy density. (B) DIC images of one iRBC (left) being detached from the adhesive CHO cell (right). The aspirating pressure increased stepwise in each pulling (scale bar = 5 μm) (C) The relationship between the contact diameter and the aspirating pressure. Each data point represents the cell-cell contact diameter when the iRBC was pulled at corresponding aspirating pressure. Data was extracted from one experiment.