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. 2018 Oct 9;8:15022. doi: 10.1038/s41598-018-33358-2

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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(A) Percentage of adhesion measured at 23 °C, 37 °C, and after 1 h or 2 h incubation at 40 °C (n = 5). (B) The adhesion force measured at 23 °C, 37 °C, and after 1 h incubation at 40 °C. (C) The initial contact diameter measured at 23 °C, 37 °C, and after 1 h incubation at 40 °C. (D) The final contact diameter before the two cells were separated, measured at 23 °C, 37 °C and after 1 h incubation at 40 °C. Each point in (B), (C) and (D) represents the adhesion force of one cell pair, and the bar represents the median value of each data set. Krauskal-Wallis Test and Dunn’s post hoc test.