Fig. 2.

MiR-204 upregulation induced human AML cell apoptosis. (A) Human AML cell lines, AML5, HL-60, Kasumi-1 and U937, were transfected with miR-204 mimics (miR-204-m, 20 nM) or mimics negative control (NC-m) for 48 h. Cell apoptosis was examined by Annexin V/PI staining using flow cytometry. (B) The percentage of apoptosis ratio was quantified. **P < 0.01 vs. control, n = 8. Cell cycle was quantified by flow cytometry. **P < 0.01 vs. control, n = 6. (C) Representative flow cytometry histograms displaying the effect of miR-204 overexpression on AML5, HL-60, Kasumi-1 or U937 cell cycle distribution. (D) Graphs correspond to the distribution of cell population in different phases.