Fig. 3.

MiR-204 negatively regulated BIRC6 expression and activated p53-dependent apoptosis pathway. (A) BIRC6 was predicted as the target gene of miR-204. The complementary sequences were shown. (B and C) Western blotting analysis of BIRC6 expression in AML cells transfected with miR-204 mimics (miR-204-m) (B) or miR-204 inhibitor (miR-204-i) (C). (D) AML cells were cotransfected with luciferase reporter containing wild-type (WT) BIRC6 3′ UTR or the mutant one, and miR-204 mimics or mimics negative control. Dual luciferase activity assay was performed to examine the luciferase activity of BIRC6 3′ UTR. **P < 0.01 vs. control, n = 6. (E and F) The protein expression of p53 and Bax in AML cells transfected with miR-204 mimics (E) or miR-204 inhibitor (F) was determined.