Figure 1.

Capsaicin induces the upregulation of TRIB3 in different cancer cell lines.

Notes: (A) Cancer cell lines vary in their TRIB3 expression levels. The protein (upper panel) and mRNA (lower panel) expression levels of TRIB3 were measured in seven different cancer cell lines. (B) AGS, MKN-45, and TMK-1 cells were seeded in a 96-well plate for 24 hours and then exposed to 0, 50, 100, 200 µM capsaicin for 48 hours. Cell viability was determined using the WST-1 reagent. n=5. *P<0.05 as the cell viability of MKN-45 was significantly different from those of AGS and TMK-1. (C) Cells were exposed to different concentrations of capsaicin and the protein levels of TRIB3 were determined. (D) MKN-45 and SiHa cells were treated with 100 µM capsaicin for 6–24 hours and the protein levels of TRIB3 were determined. All data were obtained from at least two to four independent experiments.

Abbreviation: n.s., not significant.