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. Author manuscript; available in PMC: 2019 Jul 20.
Published in final edited form as: ACS Synth Biol. 2018 Jul 10;7(7):1722–1729. doi: 10.1021/acssynbio.8b00195

Figure 3. Mutual orthogonality between p1 and p2 replication.

Figure 3.

(A) Schematics detailing strains for measuring p1 mutation rate (left, red) and measuring p2 mutation rate (right, blue). Left: p1 mutation rate reporter strains derived from AR-Y304 encode mKate2, URA3 and leu2* on p1, enabling measurement of p1 copy number and mutation rate in presence of TP-DNAP variants in trans. Right: p2 mutation rate reporter strains derived from GA-Y021 encode mKate2, URA3 and leu2* on p2, enabling measurement of p2 copy number and mutation rate in presence of TP-DNAP variants in trans. (B) Mutagenic TP-DNAP1 variants increase p1 mutation rate (red) by 380- and 870-fold, without any increase in p2 mutation rate (blue). (C) Mutagenic TP-DNAP2 variants increase p2 mutation rate by 16- and 29-fold, without a similar increase in p1 mutation rate. Error bars correspond to 95% confidence intervals calculated according to the MSS method.17–19 See Table S2 for exact mutation rate values.