Table 1. TP-DNAP2 variants with elevated mutation rates.
Entry 1 is the native mutation rate of p2 replication measured in GA-Y021, where p2-delORF1-mUL* is replicated only by the self-encoded wt TP-DNAP2. Entries 2–18 are mutation rate measurements of p2 replication in presence of 17 error-prone TP-DNAP2 hits from library screening. All per-base substitution rates were measured using leu2* fluctuation analyses in GA-Y021, where p2-delORF1-mUL* is replicated in tandem by wt TP-DNAP2 and a TP-DNAP2 variant encoded in trans on a nuclear CEN6/ARS4 plasmid. Ranges in parentheses correspond to 95% confidence intervals, determined according to the MSS method17–19.
| Entry | TP-DNAP2 variant in trans | Per-base substitution rate of p2 (x10−10) | Fold increase |
|---|---|---|---|
| 1 | None | 5.96 (3.57–8.77) | − |
| 2 | S370Q | 83.0 (62.8–105) | 13.9 |
| 3 | Y424Q | 77.5 (56.9–100) | 13.0 |
| 4 | Y424E | 73.3 (55.2–93.3) | 12.3 |
| 5 | S370P | 50.1 (32.4–70.4) | 8.4 |
| 6 | Y424K | 44.4 (32.2–58.0) | 7.5 |
| 7 | S370R | 31.9 (22.4–42.5) | 5.3 |
| 8 | Y424G | 28.8 (20.0–38.7) | 4.8 |
| 9 | S370E | 27.9 (19.7–37.2) | 4.7 |
| 10 | S370K | 26.2 (18.0–35.4) | 4.4 |
| 11 | Y424R | 19.6 (13.0–27.1) | 3.3 |
| 12 | S370L | 14.8 (9.32–21.1) | 2.5 |
| 13 | L474D | 12.2 (7.33–17.9) | 2.0 |
| 14 | F882A | 11.7 (4.92–20.4) | 2.0 |
| 15 | F882V | 10.8 (6.30–16.2) | 1.8 |
| 16 | L474A | 8.37 (4.80–12.6) | 1.4 |
| 17 | F882R | 7.32 (3.91–11.5) | 1.2 |
| 18 | L474V | 5.76 (2.88–9.33) | 1.0 |