Figure 2.

C. thermophilum Ctr2 and Ctr3 homologues rescue growth on nonfermentable medium. A, S. cerevisiae ctr1Δctr3Δ cells (strain MPY17) were transformed with the indicated plasmids and plated on rich agar medium containing either dextrose (YPD), ethanol and glycerol (YPEG), or YPEG 50 μm CuSO₄ and photographed after 2 days (YPD) or 5 days (YPEG). B, the same cells as in A were plated on minimal agar medium containing either dextrose (SC), ethanol and glycerol (SCEG), or SCEG plus the indicated final CuSO₄ concentrations and photographed after 3 days (SC-His) or 7 days (SCEG-His). C, the same cells as in A and B were inoculated into liquid YPEG or SCEG-His medium, and growth was monitored by optical absorbance.