Figure 5.

C. thermophilum Ctr3a and Ctr3b are plasma membrane–localized transporters. A, S. cerevisiae ctr1Δctr3Δ cells (strain MPY17) were transformed with plasmids expressing the indicated proteins and plated on media to assay growth. B, immunoblot from Triton X-100–solubilized protein extracts from selected transformants in A probed with anti-His₆ antibody or anti-PGK antibody. C, cells from A were visualized by fluorescence microscopy and photographed. DIC, differential interference contrast.