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. 2018 Aug 22;293(40):15483–15496. doi: 10.1074/jbc.RA118.004084

Figure 2.

Figure 2.

Bcl3 suppression induces apoptosis and reduces proliferation of OC cells. A, Bcl3 mRNA analyzed by quantitative RT-PCR in SKOV3 and OVCAR3 cells transfected with control and Bcl3 siRNA (n = 4). B, Bcl3 Western blotting in WCE prepared from SKOV3 and OVCAR3 cells transfected with control and Bcl3 siRNA. C, densitometric evaluation of Bcl3 protein levels shown in B. The Bcl3 densities were normalized to actin. Apoptosis measured by cytoplasmic nucleosome enrichment assay (D), and caspase-3 activity (E) in SKOV3 and OVCAR3 cells transfected with control or Bcl3 siRNA. Cell proliferation was measured by CellTiter 96 One Solution cell proliferation assay in SKOV3 (F) and OVCAR3 (G) cells transfected with control or Bcl3 siRNA. The values represent the mean ± S.E. of four experiments; *, p < 0.05; **, p < 0.01; ***, p < 0.001 compared with cells transfected with the corresponding control siRNA.