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. 2018 Aug 22;293(40):15483–15496. doi: 10.1074/jbc.RA118.004084

Figure 4.

Figure 4.

Bcl3 overexpression induces survival and proliferation of OC cells. A, RT-PCR of Bcl3 mRNA in SKOV3 cells transfected with CRISPR knockout (KO), overexpression (ove) plasmid, or stably transfected with Bcl3 shRNA. B, Western blotting of Bcl3 in WCE prepared from SKOV3 cells transfected with control, Bcl3 KO, Bcl3 ove plasmids, or stably transfected with Bcl3 shRNA. C, densitometric evaluation of Bcl3 protein levels shown in B; the Bcl3 densities were normalized to actin, and expressed as % compared with cells transfected with control plasmid. D, apoptosis measured by nucleosome enrichment assay in SKOV3 cells transfected with control, Bcl3 KO, Bcl3 ove plasmid, and stably transfected with Bcl3 shRNA. Cell proliferation was measured by CellTiter 96 One Solution cell proliferation assay in SKOV3 cells transfected with (E) Bcl3 KO, (F) Bcl3 ove plasmid, or (G) stably transfected with Bcl3 shRNA. The values represent the mean ± S.E. of three experiments; *, p < 0.05; **, p < 0.01; ***, p < 0.001 compared with cells transfected with the corresponding control plasmid.