Figure 6.

IFN induces Bcl3 expression, resulting in increased PD-L1 expression in OC cells. A and B, RT-PCR of Bcl3 (A) and PD-L1 (B) mRNA in SKOV3 and OVCAR3 cells incubated 48 h with increasing IFN. C, Western blotting of Bcl3 and PD-L1 in WCE of SKOV3 and OVCAR3 cells incubated 48 h with IFN. D, densitometric evaluation of Bcl3 and PD-L1 protein levels shown in C; Bcl3 and PD-L1 densities were normalized to actin, and expressed as % compared with untreated (UT) cells. E, Western blotting of Bcl3 in WCE from IFN-treated SKOV3 and OVCAR3 cells transfected with control and Bcl3 siRNA. F, PD-L1 mRNA in IFN-treated SKOV3 and OVCAR3 cells transfected with control and Bcl3 siRNA. The values represent the mean ± S.E. of three experiments.