Figure 8.

Bcl3 and p300 mediate Lys-314/315 ac-p65 occupancy at PD-L1 promoter in IFN-treated OC cells. A–C, ChIP of PD-L1 promoter occupancy by Lys-314/315 ac-p65 (A), p65 (B), and p300 (C) in SKOV3 cells transfected with control, Bcl3, and p300 siRNA, and treated 6 h with IFN (0 and 50 ng/ml). Each condition represents ∼0.25 × 10⁵ cells. The data are presented as fold-difference in occupancy of the particular protein at the particular locus compared with the human IGX1A (SA Biosciences) locus, and represent the mean ± S.E. of three experiments. Asterisks denote a statistically significant (*, p < 0.05; **, p < 0.01; ***, p < 0.001) change compared with cells transfected with control siRNA.