Figure 2.
The effects of erlin1 and erlin2 deletion on IP3R1 ERAD. A, cells were treated with 100 nm GnRH for 0–20 min, and anti-IP3R1 immunoprecipitates and input lysates were probed in immunoblots for the proteins indicated. B, quantitated IP3R1-associated ubiquitin immunoreactivity graphed as a percentage of control cell maximum (n = 4). C, cells were treated with 100 nm GnRH for 0, 30, or 60 min, and cell lysates were probed for the proteins indicated. D, quantitated IP3R1 immunoreactivity graphed as a percentage of t = 0 values (n = 4). E, [Ca2+]c in control, E1KO, and E2KO αT3 cells exposed to 100 nm GnRH (n ≥ 9). Error bars represent S.E.