Figure 1.

Urea SDS-acrylamide gel of Vc-NQR and Pa-NQR and blue native/second-dimension urea SDS-PAGE of Pa-NQR. A, subunit and flavin cofactor identification following Ni-NTA and DEAE FPLC chromatography purification. Left, Coomassie Blue–stained gel lanes of Vc-NQR and Pa-NQR. Right, unstained gel lanes carrying Vc-NQR (Vc) and Pa-NQR (Pa) exposed to UV light, using a transilluminator. B, Coomassie Blue–stained blue native gel of Pa-NQR (i); in-gel NADH dehydrogenase activity assay (ii); Coomassie Blue–stained second-dimension SDS-PAGE (iii); second-dimension SDS-PAGE exposed to UV light (iv).