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Effect of monovalent cations on Pa-NQR activity
Activity measurements were conducted in the presence of 250 μm NADH and 50 μm ubiquinone-1, with different concentrations of monovalent cations (NaCl, KCl, and CsCl). The saturation kinetics were fitted to the Michaelis–Menten equation to calculate the kcat and Ka values.
| Cation | kcat | Ka |
|---|---|---|
| s−1 | mm | |
| NaCl | 210 ± 21 | 92 ± 25 |
| KCl | 129 ± 7 | 30 ± 6 |
| CsCl | 115 ± 2 | 65 ± 3 |
